Fig. 4

Antiangiogenic and vascular-disrupting effects of plocabulin. a Inhibition of de novo angio-tube formation; HUVEC cells were seeded on top of a Matrigel layer and incubated in the presence or absence of plocabulin for 24 h; cells were stained with calcein-AM and photographed by fluorescence microscopy. b Disruption of angio-tubes; HUVECs were seeded on top of a matrigel layer, as described, and treated with different concentrations of plocabulin; cultures were stained with Calcein-AM (green) and photographed after 24 h of incubation