Fig. 3

Plocabulin inhibits the migration and invasion capabilities of HUVEC cells. a For migration experiments, HUVEC cells were seeded in serum-free medium into the upper compartment of transwell inserts and incubated at 37 °C for 24 h in the absence (WO/S) or presence of FBS 2% (NT) or the indicated concentrations of plocabulin. After removing non-migrated cells from the upper compartment, migrated cells in the lower compartment were stained with sulforhodamine B. b For invasion experiments, the porous membranes of transwell inserts were pre-coated with matrigel basement membrane (12 μg); HUVEC cells were then seeded in serum-free medium (WO/S) into the upper compartment and incubated at 37 °C for 24 h in the absence (NT) or presence of the indicated concentrations of plocabulin. After removing non-invasive cells from the upper compartment, migrated cells in the lower compartment were stained with sulforhodamine B. c Antiproliferative activity of plocabulin in HUVEC cells after 24 of incubation. Cell viability was measured by MTT assay