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Table 1 Baseline Characteristics of included studies

From: Comprehensive evaluation of NT5E/CD73 expression and its prognostic significance in distinct types of cancers

Author Tumor type Year No. of cases CD73+ No. Positive rate Test methods Definition of high expression
Hoon et al. Ovarian cancer 2012 167 117 70.10% IHC using a 4-value grade ( >0 score)*
Anna et al. Breast cancer 2012 136 101 74.30% IHC > median expression levels
Wu et al. Colorectal Cancer 2012 223 100 44.80% IHC using X-tile program to determine the cutoff value
Wu et al. Colorectal Cancer 2012 135 68 50.40% IHC using X-tile program to determine the cutoff value
Lu et al. Gastric cancer 2013 68 31 45.60% IHC semi-quantitative method >5**
Xiong et al. Gallbladder cancer 2014 108 59 54.60% IHC the percent of positively stained cells was >10 %
Martin et al. Ovarian cancer 2015 208 104 50.00% IHC highest 20% CD73 expression was used as a cutoff
Marian et al. Bladder Cancer 2015 174 46 26.40% IHC semi-quantitative 3-scale scoring system: =2***
Yu et al. Renal cell carcinoma 2015 159 75 47.20% IHC using a 4-value intensity score ( >2 score)
Zhang et al. Rectal adenocarcinoma 2015 90 47 52.20% IHC > median expression levels
Bruno et al. Prostate Cancer 2015 285 NA NA IF > median mean fluorescence intensity
Ren et al. HNSCC 2016 162 100 61.70% IHC > median expression levels
Ren et al. Oral squamous cell carcinoma 2016 113 66 58.40% IHC > 10% positively stained cells
Zhang et al. Colorectal Cancer 2016 566 283 50.00% MA > median expression levels
Yusuke et al. Non-small-cell lung cancer 2017 642 66 10.30% IHC H-scores that met or exceeded the individual cutoffs
  1. *4-value grade: CD73 expression levels were graded on a scale of 0 to 3 based on cytoplasmic and membrane staining intensity and the proportion of positive tumor cells by an expert pathologist who was blinded to the patient’s clinical records. The staining was graded as 0 if no cancer cells were reactive, 1 if staining was weakly positive in <1/3 of cancer cells, 2 if staining was weakly positive in >2/3 of cancer cells, or strongly positive in >1/3 of cancer cells, and 3 if staining was weakly positive in most cancer cells, or strongly positive in >2/3 of cancer cells. Immunohistochemical staining for CD73 in ovarian cancer tissue was classified as negative (grade 0) or positive (grade 1 to 3).
  2. **Semi-quantitative method: The percentage of positive cells was scored 0 for staining of < 1%, 1 for staining of 2%-25%, 2 for staining of 26%-50%, 3 for staining of 51%-75%, and 4 for staining > 75% of the cells examined. Staining intensity was calculated, no coloring, slightly yellow, brown yellow and tan stains were marked as 0, 1, 2 and 3. Finally, we calculated the product of staining intensity and positive cell percentage: ≤ 5 was de ned as negative and ≥ 6 as positive.
  3. ***Semi-quantitative 3-scale scoring system, score 0: no staining; score 1+: weak staining; score 2+: strong staining.
  4. H-scores were calculated by multiplying the intensity score (0, absent; 1, weak; 2, moderate; 3, strong) by the percentage of stained cells (0–100%) to yield a value of 0–300.
  5. No., number; IHC, Immunohistochemistry; IF, immunofluoresence; MA, microarray analysis; HNSCC, Head and neck squamous cell carcinoma; NA, not applicable.