Fig. 6

Epigenetic regulation of apoptosis regulatory genes in PDAC cells. a MiaPaCa2 cells were crosslinked and subjected to ChIP using H3K9me3-specific antibodies. The levels of H3K9me3-bound genomic DNA were then quantified by qPCR using primers within the − 1000 to + 1000 region relative to the BAK, BAX and BCL2L11 (Bim) promotor regions. The ChIP PCR-amplified DNA regions at BAK, BAX and BCL2L11 promoter are shown at the top panel. Input genomic DNA was used as internal controls. Column:mean; Bar:SD. b Miapaca2 cells were crosslinked and subjected to ChIP using H3K4me3-specific antibodies. The levels of H3K4me3-bound genomic DNA were then quantified by qPCR using primers within the − 1000 to + 1000 region relative to the BCL2L1 (Bcl-x), CFLAR (FLIP) and MCL1 promotor regions. The ChIP PCR-amplified DNA regions at BCL2L1, CFLAR and MCL1 promoter are shown at the top panel. Input genomic DNA was used as internal controls