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Fig. 3 | BMC Cancer

Fig. 3

From: Contrasting roles of H3K4me3 and H3K9me3 in regulation of apoptosis and gemcitabine resistance in human pancreatic cancer cells

Fig. 3

Verticillin A alters the expression levels of a panel of apoptosis regulators. a MiaPaCa2 and PANC1 cells were treated with verticillin A at the indicated concentrations for 2 days. Cytosol and mitochondrial fractions were prepared and resolved in 4–20% SDS-polyacrylamide gels, and then analyzed by Western blotting analysis using antibodies for the indicated proteins. β-actin is used as normalization control for the cytosol fractions. CoxIV is used as normalization control for the mitochondrial fractions. Western blots were cropped to improve the conciseness of the results. Gels were run and blotted under the same experimental conditions. Blots from different gels are separated by white space. b The protein band intensities were quantified using NIH image J. The cytosol protein levels were normalized as the ratio over the intensity of β-actin. The mitochondria protein levels were normalized as the ratio over the intensity of CoxIV. Column: mean; Bar: SD

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BMC Cancer

ISSN: 1471-2407

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