Fig. 4

Cell cycle/ploidy analysis on two sensitive and two resistant CRC cell lines. Cells were exposed to TAK-960 (0.1 and 1 μM) or mock treated control for 24 and 48 h, stained with Krishan’s and analyzed for ploidy by flow cytometry. a, b HCT116, 24 and 48 h respectively, c, d WIDR, 24 and 48 h respectively, e, f DLD1, 24 and 48 h respectively, g, h COLO678, 24 and 48 h respectively. 4 N * p < 0.05, **p < 0.01 > 4 N, ## p < 0.01 by unpaired t-test. N = 3