Fig. 2

Organotypic brain slice cultures maintain characteristic features of adult brain tissue. Immunohistochemical staining of 4-6 μm sections of adult murine brain slices (prepared from 350 μm vibratome sections and cultivated for four days) compared to 4-6 μm sections of whole adult murine brains. Note the comparable patterns of blood vessels, as indicated by laminin, and myelinating oligodendrocytes, as indicated by MBP staining. We observed slight activation of astrocytes (GFAP) and microglia (Iba1) within the brain slice, presumably induced by the mechanical trauma of vibratome cutting. White scale bars 100 μm, black scale bars 1 mm