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Fig. 1 | BMC Cancer

Fig. 1

From: Monitoring the responsiveness of T and antigen presenting cell compartments in breast cancer patients is useful to predict clinical tumor response to neoadjuvant chemotherapy

Fig. 1

Assessing different cell populations ex vivo in PBMCs from healthy donors and BC patients before and after chemotherapy. a Paired analysis of tumor size (area in cm2) of the patients before therapy and after three cycles of A/C chemotherapy (n = 17). b Working strategy for multi-parametric cell analysis using flow cytometry. Monocytes and lymphocytes were defined by contour plots using SSC-A vs. FSC-A. Myeloid and plasmacytoid dendritic cell (DCs) (cells HLA-DR+ Lin1/CD15- CD11c + or HLA-DR+ Lin1/CD15- CD123+ respectively) and myeloid-derived suppressor cells (MDSCs) (cells HLA-DR- Lin1/CD15- CD13+ CD33+ ± Arginase 1+), were analyzed within the monocytic cell region. Finally, the percentage of CD4+ and regulatory T cells CD4+ CD25+ CD127- FoxP3+ (Tregs) was estimated within the lymphoid cell region. c Percentage of different sub-populations ex vivo in PBMCs from healthy donors (white box n = 10) and BC patients before (gray box n = 12) and after chemotherapy (dashed box n = 12). Panels summarize the percentages of DCs populations (top panel), MDSCs (middle panel) and CD4+ and CD4+ Tregs: CD4+ CD25+ CD127- and CD4+ CD25+ CD127- FoxP3+ (middle and right panels at the bottom). Paired analysis by Wilcoxon test, *** p < 0.001. Box and whiskers graph with 10–90% of data

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BMC Cancer

ISSN: 1471-2407

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