Fig. 3
From: AZGP1 inhibits soft tissue sarcoma cells invasion and migration
Ectopic expression of AZGP1 inhibited RD cell spreading, migration and invasion. a and b The expression level of AZGP1 in STS cell lines were determined by qPCR and western blot. c and d qPCR and western blot analysis were performed to confirm ectopic expression of AZGP1 in RD cells. e Wound healing assay showed the spreading of cells was significantly retarded after AZGP1 over-expression compared with control cells. f Boyden chamber assays showed that cell migration and invasion through matrigel were remarkably suppressed in AZGP1 over-expressing cells compared with the control cells, respectively. The quantification results of migrated cells and invaded cells through matrigel are plotted in (g and h), respectively. Data in (g and h) represent the mean ± SD from three independent experiments