Fig. 1
From: PCAF-mediated acetylation of Lin28B increases let-7 biogenesis in lung adenocarcinoma H1299 cells
The effect of Lin28B knockdown on H1299 cells. a The nuclear and cytoplasmic distribution of Lin28B in H1299 cells. Nuclear (NE) and cytoplasmic (CE) extracts were immunoblotted with anti-Lin28B antibody. Lamin B: nuclear marker, GAPDH: cytoplasmic marker. b and c) The expression levels of Lin28B protein (b) and mRNA (c) in H1299 cells with Lin28B stably knocked down. Whole cell extracts (WCEs) from sh-Lin28B-1-, sh-Lin28B-2-, and sh-luc-transduced H1299 cells were subjected to gel electrophoresis and were blotted with antibodies, as shown on the right. β-actin: loading control (b); RT-qPCR. U6 snRNA was used as reference for mature miRNAs (c). d RT-qPCR analysis showing the levels of let-7a-1, let-7g and mir-16-1 in the control and Lin28B stable knockdown cells. Sh-luc: sh-RNA specific for luciferase as a negative control. In histograms, each bar represents a mean value ± S.D. from at least three independent experiments (#, P > 0.05; *p ≤ 0.05; **p ≤ 0.01)