Fig. 3
From: Mechanism of recipient cell-dependent differences in exosome uptake
Uptake of DiO-labeled A549-, HCT116- and COLO205-derived exosomes into the individual recipient cells. Exosomes were isolated from A549 (a), HCT116 (b) and COLO205 (c) cells using the ultracentrifugation method. Uptake of DiO-labeled exosomes was analyzed by immunofluorescence microscopy. Representative images are shown. Magnified images represent high-magnification images of the boxed area. Immunofluorescence intensities of DiO-labeled exosomes per cell were analyzed using ImageJ software. Data are expressed as means ± SEM (n = 3–5). *P < 0.05; **P < 0.01; NS: not significant