Fig. 2

miR-199a/b-3p down-regulates PAK4 expression in vitro. a, b MGC-803 and SGC-7901 cells were transfected with negative control (NC) or miR-199a/b-3p mimics at a final concentration of 10 or 50 nM, at 48 h post-transfection, PAK4 mRNA and protein expression was detected using real-time PCR and western blot, respectively. Data are shown as the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001