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Fig. 7 | BMC Cancer

Fig. 7

From: Docosahexaenoic acid reduces sterol regulatory element binding protein-1 and fatty acid synthase expression and inhibits cell proliferation by inhibiting pAkt signaling in a human breast cancer MCF-7 cell line

Fig. 7

Effect of DHA on 3H–thymidine incorporation by MCF-7 cells. Cells were pretreated with BSA or 60 μM BSA-bound DHA for 48 h, then with or without 10 nM E2 (a) or 1 μg/ml of insulin (b) for 24 h, then cell proliferation were determined using the 3H–thymidine incorporation assay. Two-way ANOVA followed by the Bonferroni posttest was used to compare DHA and E2/insulin effects. The levels are expressed as a fold value compared to control cells treated with BSA with no E2 or insulin stimulation. The data are presented as the mean ± S.E.M for 6 independent experiments

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