Fig. 6

Decreased latency and enhanced growth of U251-MG tumors co-expressing Survivin and mycN. a Kaplan Meier survial plot of mice xenografted with indicated differentially transduced U251-MG cells. U251-MG cells harboring simultaneous overexpression of Survivin and mycN generated tumors with significantly shorter latency than cells that only expressed oncogenic mycN. *p < 0.05. b Tumor growth curve: tumors co-expressing Survivin and mycN grew significantly faster than tumors containing mycN alone. *p < 0.05. c Paraffin-embedded tissue sections were analyzed for proliferation (Ki67) and for apoptosis (TUNEL assay). d Quantification of Ki67 positive cells and of apoptotic cells. Survivin/mycN tumors did not exhibit differences in cell proliferation compared to mycN tumors. Note that, tumors co-expressing the transgenes showed markedly decreased apoptosis. Depicted are mean values ± SD. **p < 0.01