Fig. 6

Effects of Mino and WP1066 alone or in combination on the cell survival and caspase-3 activation in U87 glioma cells. a U87 glioma cells were treated with Mino (5 or 50 μM) and WP1066 (25 μM) alone or in combination and cell survival was assessed 48 h after the treatment using the WST-1 assay. Mino plus WP1066 synergistically inhibited cell growth. ***p < 0.001 vs. control. b Western blotting analysis of cleaved caspase-3. U87 glioma cells were treated with Mino (50 μM) and WP1066 (5 μM) for the indicated times. The expression of the cleaved fragment of caspase 3 was significantly increased. c U87 glioma cells were treated with Mino (50 μM), WP1066 (5 μM), or their combination for 48 h. The expression of the cleaved fragment of caspase 3 was determined using Western blotting analysis