Fig. 4
Cell proliferation inside the zebrafish embryos at the two conditions tested. a Zebrafish embryo incubation at 34 °C, analyzed with ZFtool, yielded a proliferation index of 0.6253 (b) Zebrafish embryo incubation at 36 °C analyzed with ZFtool yielded a proliferation index of 2.4237. Images are representative of each of the conditions assayed. All images are a superposition of a fluorescence field image over a bright field image. In all panels, the left image is a 48 hpf or 0 hpi zebrafish embryo, and the right image is the same zebrafish embryo with 120 hpf or 72 hpi. Scale bar = 100 μm. c Quantization of cell proliferation inside the embryos at the two temperatures tested in each experiment (34 °C-36 °C). (nrep = 20-50, ntotal = 207, ***p < 0.01)