Fig. 2

MUC4 is overexpressed in IMPC. a Representative images of H&E and MUC4 staining by IHC of pure, mixed and metastatic IMPC. b Kaplan–Meier analysis of the probability of DFS of patients who received adjuvant trastuzumab treatment, based on the expression of MUC4 and IMPC. c Representative images of MUC4 staining of IMPC in different histological breast cancer subtypes by IHC. MUC4 expression was scored according to Workman et al. [17]. d and e Scores of MUC4 expression classified in the histological subtypes IMPC, IDC, ILC and mucinous carcinoma in a cohort of 113 invasive breast cancer samples and in the 86 HER2-positive breast cancer cohort respectively. f Log Fold Change (FC) expression (Tumor vs. Universal reference) sample distribution for MUC4 over each breast cancer subtype. *P < 0.05, **P < 0.01