Fig. 10
Inhibit plexin-A1 expression in tumor cells can influence HUVECs tube formation and migration. a-f MGC803 or HGC27 cells were divided into the shRNA knockdown group (Siplexin-A1) and control shRNA group (SiNC). HUVEC were co-culture with MGC803 (a-b) or HGC27 cells (d-e) in the matrigel coated 96-well plate. After 8 h, the tubular structures were photographed. Representative photographs (Scale bar, 100 μm) and quantitative analysis of tube area are shown in each panel (c, f). g-l: MGC803 (g-h) or HGC27 (j-k) gastric cancer cells (1 ml/well) were seeded bottom chambers at a concentration of 4 × 105 cells/ml., which were divided into the shRNA knockdown group (Siplexin-A1) and control shRNA group (SiNC). HUVECs were suspended and planted in upper chambers. After 24 h, the migrated HUVEC were photographed (Scale bar, 100 μm) and quantitative analysis are shown in each panel (i, l). Data represent mean ± SD (n = 3 for each group,*P < 0.05, **P < 0.01)