Fig. 2

MSCs pretreated with inflammatory cytokines promote angiogenesis in chicken embryonic allantoides by producing of VEGF and PDGF. MSCs were pre-stimulated by inflammatory cytokines IFN-γ and TNF-α for 12 h. a and b ELISA analysis of the protein expression of VEGF and PDGF in the supernatant of MSCs. c and d Real-time PCR analysis of the mRNA expression of VEGF and PDGF in MSCs. BVs were broken down into two classes: bole vessels and branches on the bole vessels with vessel diameters not less than 1mm defined as class I BVs; branches on the bole vessels with vessel diameters less than 1mm were defined as class II BVs. e Embryonic allantois microvessels. f Quantification of chicken embryonic allantois microvessel formation. ^* p < 0.05