Table 3 Exon-specific primer set for TP53 exons 2–10
From: First-decade patient with colorectal cancer carrying both germline and somatic mutations in APC gene
TP53 Primer Set | Sequence (5′-3′) | Position (Ref: NC_000017.11 GRCh38.p7) | Exon Coverage | Amplicon length (bp) |
|---|---|---|---|---|
TP53_I1–2-732 bp-E234-F TP53_I4–5-732 bp-E234-R | AGGGTTGGAAGTGTCTCATGCT GGGGGATACGGCCAGG | 7676668 7675937 | 2, 3, 4, | 732 |
TP53_I4–5-518 bp-E56-F TP53_I6–7-518 bp-E56-R | CTGCCGTCTTCCAGTTGCTT CACCTGGAGGGCCACTGA | 7675310 7674793 | 5, 6 | 518 |
TP53_I6–7-719 bp-E789-F TP53_I9–10-719 bp-E789-R | CCTGCTTGCCACAGGTCTC AAAAGTGAATCTGAGGCATAACTGC | 7674353 7673635 | 7,,8, 9 | 719 |
TP53_I8–9-547 bp-E910-F TP53_I10–11-547 bp-E910-R | CAGGACAAGAAGCGGTGGA GCAGGCTAGGCTAAGCTATGATGT | 7673691 7673145 | 9, 10 | 547 |