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Fig. 3 | BMC Cancer

Fig. 3

From: Melanoma cells replicate through chemotherapy by reducing levels of key homologous recombination protein RAD51 and increasing expression of translesion synthesis DNA polymerase ζ

Fig. 3

RAD51 DNA repair foci are not induced by short term cisplatin treatment of melanoma cells. A375 melanoma, MRC5v1 immortalised fibroblasts and PEO4 ovarian cancer cells growing on coverslips were treated for 24 h with 6 μM cisplatin and the number of RAD51 foci in treated and control cultures was determined by RAD51 immunofluorescence of fixed cells. Graph shows the mean number of RAD51 foci per nucleus (±SEM) for treated and control cultures. Between 40 and 60 nuclei were scored for each cell type and treatment. Representative images are shown in Additional file 1: Figure S2

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BMC Cancer

ISSN: 1471-2407

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