Fig. 6From: Evaluation of epidermal growth factor receptor signaling effects in gastric cancer cell lines by detailed motility-focused phenotypic characterization linked with molecular analysisPhosphorylation profile of components of the EGFR signaling pathway in Hs746T and MKN1 cells. Levels of pMAPK (T202/Y204, T185/Y187), pMSK1/2 (S376/S360), pRSK 1/2/3 (S380), pCREB (S133), pAKT (S473), pTOR (S2448), pFAK (Y397) and pPyk2 (Y402) were determined via proteome profiler analysis. Cells were treated for 4 h with EGF and/or cetuximab. The average phosphorylation levels were quantified using densitometric analysis. The results for bolded and underlined phospho-proteins were confirmed via Western blot analysis (see Fig. 7)Back to article page