Fig. 3

Assays to monitor cell growth and proliferation of KAIMRC1 cells. a-d Colony formation assay using soft agar. Scale bar = 400 μm . e-h 3-D spheroid formation assay using methyl cellulose. Scale bar = 400 μm . i-l Time-lapse imaging of cell proliferation. Scale bar = 200 μm . m-r Immunocytochemistry of breast cancer predictive markers. Scale bar = 20 μm . s-v Scanning electron microscopy (SEM) images of breast cancer cell lines, MCF-7, MDA MB-231 and normal breast cell line MCF-10A in comparison to KAIMRC1 cells