Fig. 1From: Insulin-like growth factor receptor and sphingosine kinase are prognostic and therapeutic targets in breast cancerImmunohistochemistry and manual scoring analysis of Australian Breast Cancer Tissue Bank patient samples. Immunohistochemistry was performed on formalin-fixed paraffin embedded breast cancer patient tissue samples (n = 236) obtained from the Australian Breast Cancer Tissue Bank (ABCTB) using antibodies to detect and measure relative levels of IGF1R, p-IGF1R and SphK1. The intensity of immunostaining was assessed by manual scoring according to standard guidelines as follows: 0 = no staining, 1 = weak staining, 2 = moderate staining and 3 = strong staining for IGF1R, p-IGF1R and SphK1Back to article page