Fig. 4
The relocalization of iASPP and Sertad1 in K562-iASPPhi, Sertad1hi and Douhi cells. a Confocal microscopy images of relocalization of iASPP and Sertad1 in K562 cells, K562-iASPPhi cells, K562-Sertad1hi cells and K562-Douhi cells immunolabeled with antibodies against iASPP (red) and Sertad1 (green), and cell nucleus stained with DAPI (blue). The maximum resolution of all the images was 8 μm. b The distribution of iASPP and Sertad1 protein in cytoplasm and nucleus. The total protein was lysed by Nuclear and Cytoplasm Protein Kit following the instructions. Actin was used for a loading control of cytoplasm protein, Histone3 for nucleus protein. (C represens cytoplasm protein, N represents nucleus protein). c Co-immunoprecipitation between P53 and iASPP was done in Sertad1hi, Sertad1low and Douhi cells. Antibody against P53 was used for IP, anti-iASPP was used for IB