Fig. 6

miR-486-5p regulates expression of PTEN and PI3K/AKT pathway proteins. Levels of the indicated PI3K/AKT pathway proteins were analyzed in cervical cancer cells by western blotting; differences in expression are represented in the bar graphs. a-b HeLa cells were transfected with miR-486-5p inhibitor sponge (Inhibitor) or control miRNA (Mock). c-d SiHa cells were transfected with miR-486-5p mimic (Mimic) or control miRNA (Mock). Data (mean±SD; n = 3) and images shown are representative of one experiment. Similar results were obtained in three independent experiments. *P < 0.05, compared with Mock