Fig. 4

miR-486-5p promotes cervical cancer cell migration and invasion in vitro. a-d Cell migration was assessed by the scratch repair (wound healing) test. Extent of wound closure indicates degree of cell migration. a-b HeLa cells transfected with miR-486-5p inhibitor sponge (Inhibitor) or control miRNA (Mock) were scratched and incubated in medium with 2% FBS for 72 h. c-d SiHa cells transfected with miR-486-5p mimic (Mimic) or control miRNA (Mock) were scratched and incubated in medium with 2% FBS for 72 h. e-h Cell invasiveness was assessed by the transwell assay. Cell invasiveness was measured by determining the number of cells that migrated through a matrigel coating in the transwell chamber. e-f HeLa cells transfected with miR-486-5p inhibitor sponge (Inhibitor) or control miRNA (Mock) were placed in the transwell chambers and allowed to incubate for 24 h. g-h SiHa cells transfected with miR-486-5p mimic (Mimic) or control miRNA (Mock) were placed in the transwell chambers and allowed to incubate for 24 h. ** P < 0.01, compared with Mock