Fig. 5

Drug-induced cell death is accentuated by miR-195. This panel shows the cell cycle profile of UACC-62 melanoma cells transfected with either miRNA-control/miR-195 (10 nM) (24 h) and treated with cisplatin (CIS-2.5 and 5 μM) or temozolomide (TMZ-50 and 250 μM) for 48 h (total time 72 h). The percentage of the cell population distributed in each cell cycle phase is indicated: G0/G1 = blue, S = green, and G2/M = pink. (a) MicroRNA-195 alone increased cell death (cells accumulated at sub G0/G1). (b-e) Treatment with drugs induces mainly arrest of UACC-62 cells in G2/M whereas the cytotoxic effects of cisplatin and temozolomide were higher when combined with miR-195 transfection, inducing cell death (sub G0/G1 cells population). Cell cycle distribution of propidium iodide (PI)-labeled cells was analyzed using FlowJo Cytometric software. Representative examples of at least three independent experiments are reported