Fig. 2
From: Transcription factor E2F1 promotes EMT by regulating ZEB2 in small cell lung cancer
The cellular morphology was changed when E2F1 was depleted in SCLC cells. a E2F1 protein was examined in 8 clones where E2F1 was knocked down by shRNA in H446 cells (H446-E2F1sh) and transiently silenced by E2F1 specific siRNA in H1688 cells (H1688-E2F1si). b Cellular morphology was changed from grain-shaped to slender and fibrous in clone-4 of H446-E2F1sh and H1688-E2F1si cells. Because shRNA vector contained eGFP, H446-E2F1sh cells were green. The magnification of H446 (panel b) was 400 X, and the magnification of H1688 (panel c) was 200 X