Oestrogen receptor beta isoform expression in sporadic colorectal cancer, familial adenomatous polyposis and progressive stages of colorectal cancer

Table 2 Sequences and properties of the primers used in the study

Gene	Primer 5′ - 3′	Amplicon length (bp)	Transcript variants	Encoded Isoforms
ESR2	F:AATTGACCACCCCGGCAAG	64	a, b, d, f, k and l	ERβ1, ERβ2 and ERβ4
Primers 1	R: TTTCCCCTCATCCCTGTCCA	64	a, b, d, f, k and l	ERβ1, ERβ2 and ERβ4
ESR2	F:GGCTAACCTCCTGATGCTCC	57	a and g	ERβ1 and ERβ5
Primers 2	R: TCCATGCCCTTGTTACTCGC	57	a and g	ERβ1 and ERβ5
ESR2 ^a	F:TCTCCTCCCAGCAGCAATCC	154	b, l and k	ERβ 2
Primers 3	R:GGTCACTGCTCCATCGTTGC	154	b, l and k	ERβ 2
PUM1	F:CACAGACACCACCTCCTTCC	73
	R:CCATTCGTGAGTCCTCCCAG	73
EIF2B1	F:ACCTGTCTTCATCCTCCCCT	71
	R:GCTGCTTTTCGCCTGCATC	71
POP4	F:TTACCTGCTTTCCCGCTGAG	114
	R:GGCTAGGAAGCTACAGCACC	114

bp: base pairs ^aThis primer sequence was obtained from ref. [22] F: forward; R: reverse

ISSN: 1471-2407