Fig. 3

NHE Activity is necessary for EGF-mediated lysosome trafficking and invasion, but not overall cell motility. a DU145 cells were treated with 0.1% DMSO, 25 μM EIPA or 10 μM Tro for 2 h prior to a 16 h stimulation with 100 ng/mL EGF. Cells were then stained for LAMP-1 (red), phalloidin (green), and DAPI (blue). Scale bar represents 30 μm, N = 3. b Represents mean lysosome distribution of 25 cells; * = p < 0.05 vs. control. c DU145 were treated with 25 μM EIPA or 100 ng/mL EGF and allowed to invade through a 1:5 dilution of Matrigel for a 48 h boyden chamber invasion assay N = 3. The number of invasive cells were counted; * = p < 0.05 vs. control. d Confluent monolayers of DU145 cells were scratched with a p200 pipette tip and treated with DMSO or 25 μM EIPA for two hours prior to treatment with or without 100 ng/mL EGF. Cells were allowed to migrate into the wound for 24 h prior to fixation with 4% PFA and phalloidin staining. Representative 4X fields are shown, N = 3. Yellow lines indicate width of the initial p200 scratch. e Quantification of wound area from data in panel D. Error bars represent standard error of the mean. *p < 0.05 vs. control. (a.u. = arbitrary units)