Fig. 3

MiR-216b decreases cyclinD1, c-myc, p-Rb and LEF1 level and enhances p21 expression. a Western blotting analysis showed that miR-216b mimics decreased the relative expression of cyclin D1, c-myc, LEF1 and phosphorylated Rb (p-Rb) but increased p21 level in HeLa cells 48 h after transfection, and miR-216b inhibitors had the opposite effect, as normalized by β-actin. * P < 0.05. b Real-time PCR analysis of cyclin D1, p21, myc and LEF1 (lymphoid enhancer-binding factor 1) at transcriptional level showed that ectopic miR-216b could significantly decrease cyclin D1, myc and LEF1 but increase p21 mRNA whereas miR-216b inhibitors had the opposite effect. * P < 0.05