Fig. 5
From: 3,6-Dihydroxyflavone regulates microRNA-34a through DNA methylation
3,6-DHF improves the expression of TET1 by repressing DNMT1 activity. a Western blots showing the levels of DNMT1 and TET1 in MDA-MB-231 cells after transfecting pcDNA3/Myc-DNMT1. b The effect of 3,6-DHF (20 μM) on the levels of TET1 after transfecting pcDNA3/Myc-DNMT1, detected by western blot analysis. c The level of TET1 binding to its own promoter in MDA-MB-231 cells as determined by a ChIP assay with anti-TET1 antibody followed by qPCR; Site-3 is a negative control. The data are presented as the mean ± SD (n = 3). d Flow chart illustrating mechanism of 3,6-DHF in regulating DNA methylation of the miR-34a promoter