Fig. 1

3,6-DHF decreases global DNA methylation levels and promotes the expression of TET1 in breast cancer cells. a MDA-MB-231 breast cancer cells were treated with 3,6-DHF (10, 20 μM). The results are expressed as a percentage of vehicle (DMSO)-treated control. b Effects of 3,6-DHF treatment (0, 5, 10, and 20 μM) for 24 h on TET1, TET2 and TET3 in MDA-MB-231 cells as detected by qRT-PCR. The data are presented as the mean ± SD (n = 3). ^* P < 0.05 compared with the MDA-MB-231 cells treated with 0 μM 3,6-DHF for 24 h. c Western blots showing levels of TET1 in MDA-MB-231 breast cancer cells. d Anti-5hmC dot blot for DNA extracted from MDA-MB-231 cells treated with 3,6-DHF