Table 1 Cytotoxicity of replication-competent Ad on carcinoma cells
Cells |  |  | IC50 values (Average ± SE) | |||
|---|---|---|---|---|---|---|
Ad5/MK | AdF35/MK | Ad5/Sur | AdF35/Sur | Ad5/COX-2 | AdF35/COX-2 | |
Pancreatic carcinoma (1 × 103 vp/cell) | ||||||
 AsPC-1 | 16.1 ± 2.7 | 9.2 ± 0.6 | 15.7 ± 0.5 | 3.6 ± 0.6 | 245 ± 41.5 | 38.8 ± 12.7 |
 PANC-1 | 36.1 ± 8.8 | 13.7 ± 6.4 | 15.9 ± 2.6 | 2.6 ± 0.2 | 244 ± 77.1 | 27.6 ± 0.6 |
 BxPC-3 | 44.6 ± 3.1 | 4.1 ± 2.0 | 64.9 ± 8.8 | 1.8 ± 0.9 | 474 ± 9.6 | 37.1 ± 7.1 |
 MIA-PaCa-2 | 12.5 ± 2.7 | 2.2 ± 0.3 | 105 ± 16.3 | 8.0 ± 0.9 | 69.6 ± 5.4 | 32.1 ± 6.2 |
Esophageal carcinoma (1 × 104 vp/cell) | ||||||
 TE-1 | 32.2 ± 2.4 | 10.1 ± 2.0 | 56.0 ± 1.5 | 9.71 ± 4.7 | 317 ± 96.5 | 18.5 ± 1.9 |
 TE-2 | 244 ± 188 | 28.6 ± 4.6 | 100 ± 32.6 | 15.1 ± 3.9 | 62.8 ± 19.9 | 46.6 ± 22.3 |
 TE-10 | 2.6 ± 1.1 | 12.3 ± 4.7 | 27.3 ± 8.6 | 3.7 ± 0.6 | 46.1 ± 6.3 | 8.1 ± 1.6 |
 TE-11 | 1.5 ± 0.3 | 3.0 ± 0.5 | 1.5 ± 0.3 | 1.1 ± 0.5 | 30.9 ± 11.8 | 7.7 ± 1.6 |
 YES-2 | 5.7 ± 2.4 | 0.3 ± 0.2 | 7.6 ± 1.7 | 1.4 ± 0.6 | 80.1 ± 18.7 | 4.0 ± 2.7 |
 YES-4 | 1.2 ± 0.8 | 10.6 ± 1.6 | 2.1 ± 0.3 | 7.1 ± 2.8 | 18.8 ± 4.7 | 5.6 ± 1.0 |
 YES-5 | 16.7 ± 6.3 | 1.5 ± 1.0 | 8.1 ± 3.1 | 5.8 ± 1.9 | 37.5 ± 7.8 | 16.8 ± 5.3 |
 YES-6 | 424 ± 85.2 | 157 ± 40.9 | 89.5 ± 21.5 | 226 ± 14.5 | 260 ± 43.1 | 40 ± 1.9 |
 T.Tn | 9.6 ± 0.9 | 1.0 ± 0.2 | 36.1 ± 1.2 | 1.1 ± 0.0 | 58.2 ± 19.0 | 6.0 ± 0.7 |
Mesothelioma (1 × 103 vp/cell) | ||||||
 NCI-H2452 | 7.18 ± 1.0 | 18.2 ± 6.2 | 43.1 ± 2.9 | 27.2 ± 9.0 | 41.6 ± 11.7 | 50.8 ± 5.8 |
 NCI-H2052 | 520.6 ± 14.8 | 143.2 ± 24.8 | 516.1 ± 11.3 | 187.2 ± 50.9 | >1000 | 524.0 ± 18.1 |
 NCI-H226 | 35.1 ± 3.5 | 59.3 ± 0.6 | 55.5 ± 1.1 | 55.7 ± 2.5 | 550.0 ± 10.0 | 531.3 ± 11.3 |
 NCI-H28 | 4.4 ± 0.4 | 5.6 ± 0.1 | 45.9 ± 21.1 | 7.1 ± 0.1 | 140.3 ± 23.7 | 36.6 ± 2.1 |
 MSTO-211H | 12.6 ± 2.2 | 0.7 ± 0.0 | 95.0 ± 19.1 | 3.9 ± 0.2 | 450.6 ± 17.2 | 10.9 ± 2.0 |