Skip to main content

Advertisement

Table 1 Cytotoxicity of replication-competent Ad on carcinoma cells

From: Cytotoxicity of replication-competent adenoviruses powered by an exogenous regulatory region is not linearly correlated with the viral infectivity/gene expression or with the E1A-activating ability but is associated with the p53 genotypes

Cells    IC50 values (Average ± SE)
Ad5/MK AdF35/MK Ad5/Sur AdF35/Sur Ad5/COX-2 AdF35/COX-2
Pancreatic carcinoma (1 × 103 vp/cell)
 AsPC-1 16.1 ± 2.7 9.2 ± 0.6 15.7 ± 0.5 3.6 ± 0.6 245 ± 41.5 38.8 ± 12.7
 PANC-1 36.1 ± 8.8 13.7 ± 6.4 15.9 ± 2.6 2.6 ± 0.2 244 ± 77.1 27.6 ± 0.6
 BxPC-3 44.6 ± 3.1 4.1 ± 2.0 64.9 ± 8.8 1.8 ± 0.9 474 ± 9.6 37.1 ± 7.1
 MIA-PaCa-2 12.5 ± 2.7 2.2 ± 0.3 105 ± 16.3 8.0 ± 0.9 69.6 ± 5.4 32.1 ± 6.2
Esophageal carcinoma (1 × 104 vp/cell)
 TE-1 32.2 ± 2.4 10.1 ± 2.0 56.0 ± 1.5 9.71 ± 4.7 317 ± 96.5 18.5 ± 1.9
 TE-2 244 ± 188 28.6 ± 4.6 100 ± 32.6 15.1 ± 3.9 62.8 ± 19.9 46.6 ± 22.3
 TE-10 2.6 ± 1.1 12.3 ± 4.7 27.3 ± 8.6 3.7 ± 0.6 46.1 ± 6.3 8.1 ± 1.6
 TE-11 1.5 ± 0.3 3.0 ± 0.5 1.5 ± 0.3 1.1 ± 0.5 30.9 ± 11.8 7.7 ± 1.6
 YES-2 5.7 ± 2.4 0.3 ± 0.2 7.6 ± 1.7 1.4 ± 0.6 80.1 ± 18.7 4.0 ± 2.7
 YES-4 1.2 ± 0.8 10.6 ± 1.6 2.1 ± 0.3 7.1 ± 2.8 18.8 ± 4.7 5.6 ± 1.0
 YES-5 16.7 ± 6.3 1.5 ± 1.0 8.1 ± 3.1 5.8 ± 1.9 37.5 ± 7.8 16.8 ± 5.3
 YES-6 424 ± 85.2 157 ± 40.9 89.5 ± 21.5 226 ± 14.5 260 ± 43.1 40 ± 1.9
 T.Tn 9.6 ± 0.9 1.0 ± 0.2 36.1 ± 1.2 1.1 ± 0.0 58.2 ± 19.0 6.0 ± 0.7
Mesothelioma (1 × 103 vp/cell)
 NCI-H2452 7.18 ± 1.0 18.2 ± 6.2 43.1 ± 2.9 27.2 ± 9.0 41.6 ± 11.7 50.8 ± 5.8
 NCI-H2052 520.6 ± 14.8 143.2 ± 24.8 516.1 ± 11.3 187.2 ± 50.9 >1000 524.0 ± 18.1
 NCI-H226 35.1 ± 3.5 59.3 ± 0.6 55.5 ± 1.1 55.7 ± 2.5 550.0 ± 10.0 531.3 ± 11.3
 NCI-H28 4.4 ± 0.4 5.6 ± 0.1 45.9 ± 21.1 7.1 ± 0.1 140.3 ± 23.7 36.6 ± 2.1
 MSTO-211H 12.6 ± 2.2 0.7 ± 0.0 95.0 ± 19.1 3.9 ± 0.2 450.6 ± 17.2 10.9 ± 2.0
  1. Respective carcinoma cells were infected with Ad at various vp/cell ratios and the cytotoxicity was tested with the WST assay. The experiments were conducted 3 times and the representative data are shown. IC50 values were estimated with CalcuSyn software. Averages and SEs are shown (n = 3)