Tartrate-resistant acid phosphatase (TRAP/ACP5) promotes metastasis-related properties via TGFβ2/TβR and CD44 in MDA-MB-231 breast cancer cells

Table 1 Analysis conditions and number of identifications for each experimental approach

	TRAP3^high vs control				TRAP shRNA
	Phosphoproteomics analysis Ultra-acidic (IPG 2.5–3.7)		Standard proteomics analysis Wide-range (IPG 3–10)		Wide-range (IPG 3–10)
	Replicate #1	Replicate #2	Replicate #1	Replicate #2
Nr. of unique peptides	6266	5771	79,410	81,851	97,153
Nr. of unique phospho-peptides	3325	3892	b	b	b
Nr. of unique proteins	1,704^a	1,968^a	8433	8447	9848
Nr. of unique genes	1,695^a	1,957^a	8309	8315	9189

^aFor phosphoproteomics analysis the reported numbers refer only to proteins found to carry phosphorylated amino acids, and their corresponding genes
^bNo phospho-peptides are reported because phosphorylation was not included as dynamic modification when searching the data

ISSN: 1471-2407