Fig. 5
Overexpression of ERβ2 and ERβ5 increased ovarian cancer cell migration and invasion. ERβ5-mediated ovarian cancer cell migration and invasion involved FAK activation. a Immunoblot analyses of exogenous His-tagged ERβ2 and ERβ5, p-FAK Tyr397, FAK, p-c-Src Tyr416 and c-Src in ES-2 (left panel) and OVCA420 (right panel) cells stably transfected with His-tagged ERβ2, ERβ5 or control vector. b Wound healing assay and c in vitro migration and invasion assays in ES-2 and OVCA420 cells overexpressing ERβ2 and ERβ5. Upper panel: representative images of migrating or invading cells. Lower panel: Cell migration or invasion presented as percentage of control; n = 3; *, p < 0.05. d Left panel: immunoblot analysis on p-FAK Tyr397 and FAK in ES-2 and OVCA420 cells overexpressing ERβ5 in the presence or absence of FAK inh 14 or water (vehicle). Right panel: in vitro migration and invasion assays in ERβ5 overexpressing ES-2 and OVCA420 cells in the presence or absence of FAK inh 14 or water. Cell migration or invasion presented as percentage of control; n = 3; *, p < 0.05