Fig. 2

Effect of depletion of ABHD5, DGAT1, ACAT1 and ATGL on LNCaP cells: a western blot analysis 72 h after transfection with target and nontarget (NT) siRNA, the siRNA treatments are written on top of the blots and western blot (WB) primary antibodies used are mentioned on the left of each blot. b and c Oil red and bodipy staining performed 72 h after the siRNA transfection, the specific siRNA treatments are mentioned on top of the figures. d An MTS based assay showing relative growth 72 h after transfection with respective siRNAs. Assay was done in octuplets, the dotted line represents 50% growth inhibition. e Clonogenic assay showing number of colonies 16 days after respective siRNA transfection. All the assays were performed at least three times, representative experiments have been shown