Fig. 3

Antigen-specific cytokine production of receptor-transfected γ/δ T cells in comparison to CD8⁺ T cells. The different T-cell conditions were expanded as mentioned above (Fig. 1). After that, these cells were electroporated with RNA coding for a gp100/HLA-A2-specific TCR a or with RNA encoding a MCSP-specific CAR b. T cells electroporated without RNA (mock) were used as controls. Four hours after electroporation, T cells were co-incubated over night with human tumor cell lines at a 1:1 ratio. Induced cytokine secretion was quantified in the supernatant with a Cytometric Bead Array (CBA). Concentrations of IL-2, TNF, and IFNγ are depicted [ng/ml]; please note the different scales. Data represent means ± SEM from 7 to 10 independent experiments. P-values calculated by unpaired Student’s t-test are presented in Additional file 1: Table S3. a The cytokine secretion of gp100 TCR-RNA-transfected T cells was characterized in response to co-culture with the, either unloaded or gp100-peptide (HLA-A2 restricted) pulsed, human melanoma cell lines Mel526 and A375M. b The quantity of cytokines produced by MSCP CAR-RNA-transfected T cells was measured ensuing co-culture with the human lymphoma cell line T2.A1, and the melanoma cell lines Mel526 and A375M