Fig. 4

Angpt1 deficiency enhances initial stages of distant metastasis. a Quantification of B16F10 cells in the lungs 24 h after tail vein injection in WT (n = 6) and Angpt1 ^Δ/Δ mice (n = 6). b Quantification of B16F10 cells in the lungs 4 h after tail vein injection in WT and Angpt1 ^Δ/Δ mice (n = 4–5). c Representative fluorescent image of lung from WT and Angpt1 ^Δ/Δ mice 4 h after tail vein injection of GFP positive B16F10 cells. d Leakage experiments with cadaverin in WT and Angpt1 ^Δ/Δ mice, with and without tail vein injection of B16F10 cells. e Measurement of cross-sectional area of capillaries from micrographs of lungs from WT (n = 4) and Angpt1 ^Δ/Δ (n = 4) mice. f Quantification of microspheres in lungs 4 h after tail vein injection in WT (n = 5) and Angpt1 ^Δ/Δ (n = 5) mice**p < 0.01, ***p < 0.001. g Western blotting for FAK phosphorylation at Tyr397 in WT and Angpt1 ^Δ/Δ mice, with and without tail vein injection of B16F10 cells. h Quantification of B16F10 cell attachment to HUVECs with or without pre-treatment with Angpt1