Fig. 5

ERK/JNK/AP1 signaling mediates the effect of Trop2 on MMP2 expression. a Comparison of AP1 reporter activity in indicated cells. The luciferase reporter showed decreased activation of AP1 reporter in Trop2 knocked-down K1 and 8505C cells. The data are reported as mean ± SD of three independent experiments. *, P < 0.05. b Overexpression of Trop2 promoted the AP1 reporter activity. The data are reported as mean ± SD of three independent experiments. *, P < 0.05. c and d AP1 ODNs significantly inhibited the effects of Trop2 on MMP2 expression (c) and MMP2 promoter reporter activity (d). The data are reported as mean ± SD of three independent experiments. *, P < 0.05. e WB analysis showed that deregulated expression of Trop2 significantly altered the phosphorylation of ERK and JNK. f and g Cells were treated with a concentration of 10 μM PD98059, 10 μM SP600125, or 10 μM SB203580 for 24 h. After incubation, MMP2 mRNA level was determined by qRT-PCR (f) and AP1 reporter activity was assessed by Dual-Luciferase reporter assays (g). The data are reported as mean ± SD of three independent experiments. *, P < 0.05