Fig. 3

Over-expression of Trop2 promotes the invasion and migration of thyroid cancer cells. a Overexpression of Trop2 in FTC-133 thyroid cancer cells was analyzed. The protein levels of Trop2 in indicated cells were assessed by WB and α-tubulin was used as a loading control. b Overexpression of Trop2 increased the invasive (with Matrigel) and migratory (without Matrigel) capability of FTC-133 cells. c The histogram showed the invading and migrating cells per field. The data are reported as mean ± SD of three independent experiments. *, P < 0.05. d Wound healing analyses of indicated cells. Streaks were created with a tip, and the representative phase-contrast images of the extent of cell migration into the wounded area at 24 h are shown. Experiments were repeated for three times