Fig. 4

DIP2C knockout enhances cell motility and alters expression of EMT and cancer stem cell markers. a Quantification of the EMT regulator ZEB1, and EMT markers VIM, CDH1 and CDH2 by RT-qPCR. Mean expression for DIP2C ^−/− #1-1, and #1-2 and DIP2C ^+/− #1 and #2 is shown respectively. Error bars - min and max relative quantity (RQ). b Western blot for ZEB1. Predicted molecular weight 124 kDa. c Quantification of CD44 and CD24, markers for breast cancer stem-like cell properties, by RT-qPCR. Mean expression for DIP2C ^−/− #1-1, and #1-2 and DIP2C ^+/− #1 and #2 is shown respectively. Error bars - min and max relative quantity (RQ). d Scratch assay showing the wound closing ability for DIP2C ^−/− #1-1. The open wound area was determined by comparison of cell confluency at time points 0 and 48 h after scratching of the surface. Error bars SD. RKO n = 5 images, DIP2C ^−/− n = 4 images. Representative micrographs are shown for both time points, cell borders were traced manually to enhance visualization and were not part of the analysis. The experiment was repeated three times. (*P < 0.05; **P < 0.01; ***P < 0.001; two-tailed Student’s t-test)