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Fig. 6 | BMC Cancer

Fig. 6

From: Tamoxifen reverses epithelial–mesenchymal transition by demethylating miR-200c in triple-negative breast cancer cells

Fig. 6

TAM reversed EMT by inhibiting the expression of DNMT1/DNMT3a. a, Western blot analysis showed the expression of DNMT1 and DNMT3a in indicated cells. b, Western blot analysis showed the expression of DNMT1 and DNMT3a in MCF-7/ADR and MDA-MB-231 cells treated with or without TAM for 48 h. c, MCF-7/ADR cells were transfected with either NC or si-DNMT1 or si-DNMT3a or si-DNMT1 and si-DNMT3a for 48 h; Relative expressions of miR-200c were determined by realtime-PCR in indicated cells. d, Western blot analysis showed the expression of E-cadherin and Vimentin in double knocked (si-DNMT1 and si-DNMT3a) MCF-7/ADR cells. All experiments were repeated 3 times. Results are expressed as mean ± SD (n = 3). * P < 0.05

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