Fig. 2

TAM reversed EMT in mesenchymal TNBC cells. a, Morphology showed by electron microscope at high magnification(×40) in the indicated cells treated with or without TAM (5 μmol/L for 48 h). b, Western blot analysis showed the expression of E-cadherin and Vimentin in the indicated cells treated with or without TAM (5 μmol/L for 48 h). c, Immunofluorescence analysis showed the expression of E-cadherin (red), Vimentin (green) in the indicated cells treated with or without TAM (5 μmol/L for 48 h). Image were photographed by electron microscope at high magnification(×40). d, Migration ability of indicated cells treated with or without TAM were subjected to migration assay, the numbers of migration cells were represented by mean of three individual experiments. e, Migration ability of indicated cells treated with or without TAM were subjected to lung metastasis focals and metastasis rate; HE staining of lung tissue from mice were photographed by electron microscope at high magnification(×40). up, lung metastasis focal of breast cancer; down, normal lung sample without malignancy