Fig. 4

Point mutation detection and aCGH analysis using a single-cell WGA products that passed (QC-pass) or failed (QC-fail) the quality control step, b) 5 and 10-cell WGA products with passing QC, and c unamplified, batch genomic DNA. Single-cell WGA products (QC-fail vs. QC-pass) exhibit significantly different downstream analyses results despite using cells isolated from a clonally expanded cell line. The single-cell WGA product with the passing QC (QC-pass) generated Sanger sequencing and aCGH results that more closely resembled that of the unamplified batch gDNA compared to the single-cell WGA product failing the QC (QC-fail). Red arrows signify the areas of alteration that were detected in QC-pass and batch gDNA, but not in QC-fail. d aCGH quality metrics (DLRS and signal-to-noise ratio) for 1, 5 and 10-cell WGA products and batch gDNA. Improved DLRS values were associated with WGA products that passed the QC step as well as using an increasing number of starting cellular input