Fig. 6

Muskelin associates with HDAC6 and downregulation of Rmnd5A and muskelin affects α-tubulin acetylation. a HDAC6 associates with Muskelin independently of RanBPM. Left, whole cell extracts were prepared from control or RanBPM shRNA Hela cells and immunoprecipitated with either HDAC6 or IgG. Immunoprecipitates were analyzed by western blot with a muskelin antibody. HDAC6 immunoprecipitation was verified using an HDAC6 antibody. Input, 5% input extract. Right, quantification of relative amounts of co-immunoprecipitated muskelin normalized to immunoprecipitated HDAC6. Results are averaged from three different experiments with error bars indicating SEM. P < 0.05 (*). b Whole cell extracts were prepared from Control or RanBPM shRNA HEK cells and analyzed as described above. c Whole cell extracts were prepared from HeLa cells transfected with control, Rmnd5A, or Muskelin siRNA. Extracts were prepared either 72 or 144 h after transfection and analyzed by western blot with the indicated antibodies. Right, quantification of relative amounts of acetylated α-tubulin normalized to total α-tubulin levels. Results are averaged from at least three different experiments, with error bars indicating SD. P < 0.005 (**)