Fig. 4
From: Regulation of early growth response 2 expression by secreted frizzled related protein 1
The effect of rSFRP1 treatment on M1 and M2 polarization in Sfrp1 −/− dervived macrophages. Macrophages were isolated from spleens derived from Sfrp1 −/− mice, treated in the presence and absence of rSRP1, and stimulated with either TGF- β1 or LPS (b) for 24 h. M2 polarization was evaluated by the mRNA expression of Egr2 and M1 polarization was evaluated by Grp18 mRNA expression. The results shown represent experiments performed in duplicate and normalized to the amplification of Actb mRNA. Bars represent mean ± SEM of the fold change with respect to control mice. *p < 0.05, **p < 0.01 (significantly different from vehicle treated macrophages using student’s t-test)