Fig. 1

SFRP1 alters the expression of EGR2 in human and mouse mammary epithelial cells and tissues. a Total RNA was isolated TERT-pSUPER and TERT-siSFRP1 cell lines (left panel) and from human explant cultures treated in the absence and presence of rSFRP1 (right panel) for real-time PCR analysis of EGR2 mRNA expression. b Total RNA was isolated MCF7-PCDNA and MCF7-SFRP1 cell lines (left panel) and MCF7 cells treated in the absence and presence of rSFRP1 (right panel) for real-time PCR analysis of EGR2 mRNA expression. c Total RNA was isolated from the mammary glands of 10 wk. virgin control and Sfrp1 ^−/− female mice (left panel) and mouse mammary epithelial cells derived from control and Sfrp1 ^−/− mice (right panel) for real-time PCR analysis of Egr2 expression. Bars represent mean ± SEM EGR2/ACTB and are expressed as relative expression of control groups. *p < 0.05, **p < 0.01, ***p < 0.01 (significantly different from control using student’s t-test)