Fig. 2

Isolated tumor cells in LPSNs that were detected by immunohistochemical staining. The second half of each LPSN sample was cut into slices 4 μm thick and these slices were immunohistochemically stained using AE1/AE3 as the primary antibody. Representative cases are shown. All LPSN metastases that were detected using RT-PCR were assayed in isolated tumor cells that are indicated with a brown-colored cell membrane